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3D ultrastructural visualization of mitosis fidelity in human cells using serial block face scanning electron microscopy (SBF-SEM)
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Ferrandiz, Nuria and Royle, Stephen J. (2023) 3D ultrastructural visualization of mitosis fidelity in human cells using serial block face scanning electron microscopy (SBF-SEM). Bio-protocol, 13 (13). e4708. doi:10.21769/BioProtoc.4708 ISSN 2331-8325.
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WRAP- 3D-ultrastructural-visualization-mitosis-fidelity-human-cells-using-serial-block-face-scanning-electron-microscopy-(SBF-SEM)-2023.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (980Kb) | Preview |
Official URL: https://doi.org/10.21769/BioProtoc.4708
Abstract
Errors in chromosome segregation during mitosis lead to chromosome instability, resulting in an unbalanced number of chromosomes in the daughter cells. Light microscopy has been used extensively to study chromosome missegregation by visualizing errors of the mitotic spindle. However, less attention has been paid to understanding spindle function in the broader context of intracellular structures and organelles during mitosis. Here, we outline a protocol to visualize chromosomes and endomembranes in mitosis, combining light microscopy and 3D volume electron microscopy, serial block-face scanning electron microscopy (SBF-SEM). SBF-SEM provides high-resolution imaging of large volumes and subcellular structures, followed by image analysis and 3D reconstruction. This protocol allows scientists to visualize the whole subcellular context of the spindle during mitosis.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QH Natural history | |||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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SWORD Depositor: | Library Publications Router | |||||||||
Library of Congress Subject Headings (LCSH): | Chromosomes -- Analysis, DNA damage, Mitosis, Endoplasmic reticulum, Electron microscopy -- Technique, Scanning electron microscopy | |||||||||
Journal or Publication Title: | Bio-protocol | |||||||||
Publisher: | Bio-protocol LLC | |||||||||
ISSN: | 2331-8325 | |||||||||
Official Date: | 5 July 2023 | |||||||||
Dates: |
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Volume: | 13 | |||||||||
Number: | 13 | |||||||||
Article Number: | e4708 | |||||||||
DOI: | 10.21769/BioProtoc.4708 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Date of first compliant deposit: | 20 September 2023 | |||||||||
Date of first compliant Open Access: | 20 September 2023 | |||||||||
RIOXX Funder/Project Grant: |
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