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Data for Cryopreservation of liver-cell spheroids with macromolecular cryoprotectants
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Bissoyi, Akalabya, Tomás, Ruben M. F., Gao, Yanan, Guo, Qiongyu and Gibson, Matthew I. (2023) Data for Cryopreservation of liver-cell spheroids with macromolecular cryoprotectants. [Dataset]
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Official URL: https://doi.org/10.1021/acsami.2c18288
Abstract
Spheroids are a powerful tool for basic research and to reduce or replace in vivo (animal) studies but are not routinely banked nor shared. Here, we report the successful cryopreservation of hepatocyte spheroids using macromolecular (polyampholyte) cryoprotectants supplemented into dimethyl sulfoxide (DMSO) solutions. We demonstrate that a polyampholyte significantly increases post-thaw recovery, minimizes membrane damage related to cryo-injury, and remains in the extracellular space making it simple to remove post-thaw. In a model toxicology challenge, the thawed spheroids matched the performance of fresh spheroids. F-actin staining showed that DMSO-only cryopreserved samples had reduced actin polymerization, which the polyampholyte rescued, potentially linked to intracellular ice formation. This work may facilitate access to off-the-shelf and ready-to-use frozen spheroids, without the need for in-house culturing. Readily accessible 3-D cell models may also reduce the number of in vivo experiments.
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||||||||||||||||||||||||||||
Type of Data: | spreadsheet data | ||||||||||||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Cytology, Cells -- Cryopreservation, Cryopreservation of organs, tissues, etc., Polymers, Stem cell, Biological assay, Macromolecules | ||||||||||||||||||||||||||||||||||||
Publisher: | University of Warwick, Department of Chemistry | ||||||||||||||||||||||||||||||||||||
Official Date: | 18 January 2023 | ||||||||||||||||||||||||||||||||||||
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Status: | Peer Reviewed | ||||||||||||||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||||||||||||||
Media of Output (format): | .xlsx | ||||||||||||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||||||||||||||
Description: | Dataset to support an article published in ACS Applied Materials & Interfaces. |
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Date of first compliant deposit: | 16 August 2023 | ||||||||||||||||||||||||||||||||||||
Date of first compliant Open Access: | 16 August 2023 | ||||||||||||||||||||||||||||||||||||
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