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Detection of insecticide resistance markers in Anopheles funestus from the Democratic Republic of the Congo using a targeted amplicon sequencing panel
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Acford-Palmer, Holly, Campos, Monica, Bandibabone, Janvier, N’Do, Sévérin, Bantuzeko, Chimanuka, Zawadi, Bertin, Walker, Thomas, Phelan, Jody E., Messenger, Louisa A., Clark, Taane G. and Campino, Susana (2023) Detection of insecticide resistance markers in Anopheles funestus from the Democratic Republic of the Congo using a targeted amplicon sequencing panel. Scientific Reports, 13 (1). 17363. doi:10.1038/s41598-023-44457-0 ISSN 2045-2322.
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Official URL: https://doi.org/10.1038/s41598-023-44457-0
Abstract
Vector control strategies have been successful in reducing the number of malaria cases and deaths globally, but the spread of insecticide resistance represents a significant threat to disease control. Insecticide resistance has been reported across Anopheles (An.) vector populations, including species within the An. funestus group. These mosquitoes are responsible for intense malaria transmission across sub-Saharan Africa, including in the Democratic Republic of the Congo (DRC), a country contributing > 12% of global malaria infections and mortality events. To support the continuous efficacy of vector control strategies, it is essential to monitor insecticide resistance using molecular surveillance tools. In this study, we developed an amplicon sequencing (“Amp-seq”) approach targeting An. funestus, and using multiplex PCR, dual index barcoding, and next-generation sequencing for high throughput and low-cost applications. Using our Amp-seq approach, we screened 80 An. funestus field isolates from the DRC across a panel of nine genes with mutations linked to insecticide resistance (ace-1, CYP6P4, CYP6P9a, GSTe2, vgsc, and rdl) and mosquito speciation (cox-1, mtND5, and ITS2). Amongst the 18 non-synonymous mutations detected, was N485I, in the ace-1 gene associated with carbamate resistance. Overall, our panel represents an extendable and much-needed method for the molecular surveillance of insecticide resistance in An. funestus populations.
Item Type: | Journal Article | ||||||
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Subjects: | Q Science > QL Zoology Q Science > QR Microbiology R Medicine > RA Public aspects of medicine |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
SWORD Depositor: | Library Publications Router | ||||||
Library of Congress Subject Headings (LCSH): | Anopheles , Anopheles -- Insecticide resistance -- Congo (Democratic Republic) , Vector control , Malaria -- Prevention | ||||||
Journal or Publication Title: | Scientific Reports | ||||||
Publisher: | Nature Publishing Group UK | ||||||
ISSN: | 2045-2322 | ||||||
Official Date: | 13 October 2023 | ||||||
Dates: |
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Volume: | 13 | ||||||
Number: | 1 | ||||||
Article Number: | 17363 | ||||||
DOI: | 10.1038/s41598-023-44457-0 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 4 December 2023 | ||||||
Date of first compliant Open Access: | 4 December 2023 |
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