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A monomeric StayGold fluorescent protein
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Ivorra-Molla, Esther, Akhuli, Dipayan, McAndrew, Martin B. L., Scott, William, Kumar, Lokesh, Palani, Saravanan, Mishima, Masanori, Crow, Allister and Balasubramanian, Mohan K. (2023) A monomeric StayGold fluorescent protein. Nature Biotechnology . doi:10.1038/s41587-023-02018-w ISSN 1087-0156. (In Press)
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Official URL: http://doi.org/10.1038/s41587-023-02018-w
Abstract
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric.
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | Q Science > QP Physiology | ||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Green fluorescent protein, Proteins -- Analysis, Fluorescent polymers | ||||||||||||||||||
Journal or Publication Title: | Nature Biotechnology | ||||||||||||||||||
Publisher: | Nature Publishing Group | ||||||||||||||||||
ISSN: | 1087-0156 | ||||||||||||||||||
Official Date: | 2023 | ||||||||||||||||||
Dates: |
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DOI: | 10.1038/s41587-023-02018-w | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | In Press | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Copyright Holders: | © The Author(s) 2023 | ||||||||||||||||||
Date of first compliant deposit: | 12 December 2023 | ||||||||||||||||||
Date of first compliant Open Access: | 14 December 2023 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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