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Molecular characterisation of intracellular nanovesicles
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Moore, Daniel James (2023) Molecular characterisation of intracellular nanovesicles. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b3980915
Abstract
Tumour Protein D54 (TPD54/TPD52L2) is a member of the TPD52-like protein family and has an undefined role in cells that can lead to tumorigenesis upon dysregulation. It has been shown previously that TPD54 (and other TPD52-like proteins) localise to a novel class of trafficking vesicles in cells, termed intracellular nanovesicles (INVs). These vesicles are characterised by their small size, around 30nm, which allows them to diffuse rapidly within the cell to transport materials between membrane bound compartments without using directed transport. INVs are also characterised by a broad set of associated proteins, which suggest that collectively, they are a diverse class of trafficking vesicle. The molecular function of TPD52-like proteins on the surface of INVs, as well as the function of INVs themselves, is not yet known. Here, I aimed to characterise the structure and function of TPD54 biophysically and biochemically to determine how TPD54 binds to vesicles as well as potential activities it may perform at the vesicle surface. In addition to this, I used proteomic approaches to determine INV proteins in order to better understand how cells use INVs and on which trafficking pathways they are associated. This analysis revealed at least three large subclasses of vesicle in non-neuronal cells: ATG9 vesicles, synaptic-like vesicles and a third class of vesicles with proteins that are largely uncharacterised. This work underlines the diversity in composition and function for this class of trafficking vesicle.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QH Natural history R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
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Library of Congress Subject Headings (LCSH): | Carcinogenesis, Coated vesicles, Biological transport | ||||
Official Date: | April 2023 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | Warwick Medical School | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Royle, Stephen J. | ||||
Sponsors: | Medical Research Council (Great Britain) | ||||
Format of File: | |||||
Extent: | xi, 116, 17 pages : colour illustrations | ||||
Language: | eng |
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