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Functional differences among H3K27me3 histone demethylases in Arabidopsis thaliana
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Pellegrini, Lorenzo (2023) Functional differences among H3K27me3 histone demethylases in Arabidopsis thaliana. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b3985952
Abstract
Histone demethylases REF6 and ELF6 share strikingly similar protein structures, yet they exhibit opposing phenotypes in plants. The molecular basis for this paradox has remained elusive. In this study, we investigated the evolutionary divergence and functional diversification of REF6 and ELF6 to better understand their contrasting roles in plant development and regeneration. Our phylogenetic analysis revealed that these demethylases diverged during the emergence of flowering plants, concomitant with the appearance of distinct roles in gene regulation. Further investigation through domain swapping experiments demonstrated that the Jmj catalytic domains of REF6 and ELF6 confer distinct functional properties, leading to differential effects on gene expression and chromatin dynamics in plants. Surprisingly, when expressed in human cells, the differences between REF6 and ELF6 became even more pronounced, emphasising the functional divergence between these paralogs. Moreover, our study revealed contrasting effects of REF6 and ELF6 on plant regeneration, providing new insights into their roles in tissue differentiation and organogenesis. Collectively, our findings unveil the molecular underpinnings of the opposing phenotypes of REF6 and ELF6, shedding light on their evolutionary divergence, functional diversification, and unique roles in plant development and regeneration.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QH Natural history Q Science > QK Botany |
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Library of Congress Subject Headings (LCSH): | Arabidopsis thaliana -- Genetics, Plant proteins, Gene expression, Regeneration (Botany), Growth (Plants), Histones, Phenotype | ||||
Official Date: | April 2023 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | School of Life Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Gutierrez-Marcos, José F. | ||||
Sponsors: | Midlands Integrative Biosciences Training Partnership | ||||
Format of File: | |||||
Extent: | viii, 187 pages : illustrations | ||||
Language: | eng |
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