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Comparative lipidomics profiling of human atherosclerotic plaques
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Stegemann, C., Drozdov, I., Shalhoub, J., Humphries, Julia, Ladroue, Christophe, Didangelos, Athanasios, Baumert, M., Allen, M., Davies, A. H., Monaco, C. (Claudia), Smith, Alun H., Xu, Qingbo and Mayr, Manuel (2011) Comparative lipidomics profiling of human atherosclerotic plaques. Circulation: Cardiovascular Genetics, Vol.4 (No.3). pp. 232-242. doi:10.1161/CIRCGENETICS.110.959098 ISSN 1942-325X.
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Official URL: http://dx.doi.org/10.1161/CIRCGENETICS.110.959098
Abstract
Background-We sought to perform a systematic lipid analysis of atherosclerotic plaques using emerging mass spectrometry techniques.
Methods and Results-A chip-based robotic nanoelectrospray platform interfaced to a triple quadrupole mass spectrometer was adapted to analyze lipids in tissue sections and extracts from human endarterectomy specimens by shotgun lipidomics. Eighteen scans for different lipid classes plus additional scans for fatty acids resulted in the detection of 150 lipid species from 9 different classes of which 24 were detected in endarterectomies only. Further analyses focused on plaques from symptomatic and asymptomatic patients and stable versus unstable regions within the same lesion. Polyunsaturated cholesteryl esters with long-chain fatty acids and certain sphingomyelin species showed the greatest relative enrichment in plaques compared to plasma and formed part of a lipid signature for vulnerable and stable plaque areas in a systems-wide network analysis. In principal component analyses, the combination of lipid species across different classes provided a better separation of stable and unstable areas than individual lipid classes.
Conclusions-This comprehensive analysis of plaque lipids demonstrates the potential of lipidomics for unraveling the lipid heterogeneity within atherosclerotic lesions. (Circ Cardiovasc Genet. 2011; 4: 232-242.)
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QP Physiology R Medicine > RC Internal medicine |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Statistics | ||||
Library of Congress Subject Headings (LCSH): | Mass spectrometry, Lipids, Atherosclerosis, Atherosclerotic plaque | ||||
Journal or Publication Title: | Circulation: Cardiovascular Genetics | ||||
Publisher: | Lippincott Williams & Wilkins | ||||
ISSN: | 1942-325X | ||||
Official Date: | June 2011 | ||||
Dates: |
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Volume: | Vol.4 | ||||
Number: | No.3 | ||||
Page Range: | pp. 232-242 | ||||
DOI: | 10.1161/CIRCGENETICS.110.959098 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | British Heart Foundation , Oak Foundation |
Data sourced from Thomson Reuters' Web of Knowledge
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