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Inhibition of Akt activity and calcium channel function coordinately drive cell-cell fusion in the BeWO choriocarcinoma placental cell line
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Vatish, Manu, Tesfa, Lydia, Grammatopoulos, Dimitris, Yamada, Eijiro, Bastie, Claire C. and Pessin, Jeffrey E. (2012) Inhibition of Akt activity and calcium channel function coordinately drive cell-cell fusion in the BeWO choriocarcinoma placental cell line. PLoS ONE, Vol.7 (No.1). e29353. doi:10.1371/journal.pone.0029353 ISSN 1932-6203.
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Official URL: http://dx.doi.org/10.1371/journal.pone.0029353
Abstract
To establish a simple and quantitative live cell fusion assay for placental syncytialization, we generated stable GFP and dsRed expressing fusogenic BeWo cell lines. Fluorescent Activated Cell Sorting was shown to provide a quantitative determination of forskolin (cAMP-mediated) fusion in a time and concentration dependent manner consistent with the increased secretion of beta human chorionic gonadotrophin (β-HCG) and appearance of multi-nucleated cells. Analyses of the fusion process demonstrated that in addition to increased cAMP levels, simultaneous reduction of intracellular calcium and inhibition of Type 1 phosphatidylinositol 3 kinase (PI3K)/Akt signaling also resulted in cell fusion. Although individual blockade of calcium channel function or PI3K/Akt signaling was without effect, the combination with forskolin resulted in a potentiation of cell fusion. These data demonstrate syncytialization is a complex process that depends upon the regulation of distinct signaling inputs that function in concert with each other.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QP Physiology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Reproductive Health ( - until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Cell hybridization, Choriocarcinoma, Placenta -- Physiology | ||||
Journal or Publication Title: | PLoS ONE | ||||
Publisher: | PLOS | ||||
ISSN: | 1932-6203 | ||||
Official Date: | 19 January 2012 | ||||
Dates: |
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Volume: | Vol.7 | ||||
Number: | No.1 | ||||
Page Range: | e29353 | ||||
DOI: | 10.1371/journal.pone.0029353 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 19 December 2015 | ||||
Date of first compliant Open Access: | 19 December 2015 | ||||
Funder: | National Institutes of Health (U.S.) (NIH), United States-United Kingdom Educational Commission, Winston Churchill Memorial Trust | ||||
Grant number: | DK082694 (NIH), DK020541 (NIH), DK081412 (NIH) |
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