Tesfaye, Mesfin, Silverstein, Kevin AT., Nallu, Sumitha, Wang, Lin, Botanga, Christopher J., Gomez, S. Karen, Costa, Liliana M., Harrison, Maria J., Samac, Deborah A., Glazebrook, Jane, Katagiri, Fumiaki, Gutierrez-Marcos, José F. and VandenBosch, Kathryn A. (2013) Spatio-temporal expression patterns of Arabidopsis thaliana and Medicago truncatula defensin-like genes. PLoS One, Volume 8 (Number 3). Article no. e58992. doi:10.1371/journal.pone.0058992 ISSN 1932-6203.
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Abstract
Plant genomes contain several hundred defensin-like (DEFL) genes that encode short cysteine-rich proteins resembling defensins, which are well known antimicrobial polypeptides. Little is known about the expression patterns or functions of many DEFLs because most were discovered recently and hence are not well represented on standard microarrays. We designed a custom Affymetrix chip consisting of probe sets for 317 and 684 DEFLs from Arabidopsis thaliana and Medicago truncatula, respectively for cataloging DEFL expression in a variety of plant organs at different developmental stages and during symbiotic and pathogenic associations. The microarray analysis provided evidence for the transcription of 71% and 90% of the DEFLs identified in Arabidopsis and Medicago, respectively, including many of the recently annotated DEFL genes that previously lacked expression information. Both model plants contain a subset of DEFLs specifically expressed in seeds or fruits. A few DEFLs, including some plant defensins, were significantly up-regulated in Arabidopsis leaves inoculated with Alternaria brassicicola or Pseudomonas syringae pathogens. Among these, some were dependent on jasmonic acid signaling or were associated with specific types of immune responses. There were notable differences in DEFL gene expression patterns between Arabidopsis and Medicago, as the majority of Arabidopsis DEFLs were expressed in inflorescences, while only a few exhibited root-enhanced expression. By contrast, Medicago DEFLs were most prominently expressed in nitrogen-fixing root nodules. Thus, our data document salient differences in DEFL temporal and spatial expression between Arabidopsis and Medicago, suggesting distinct signaling routes and distinct roles for these proteins in the two plant species.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics |
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Science > Statistics |
| Library of Congress Subject Headings (LCSH): | Plant genetics, Plant physiology -- Genetic aspects, Arabidopsis, Plant genomes, Genomes |
| Journal or Publication Title: | PLoS One |
| Publisher: | Public Library of Science |
| ISSN: | 1932-6203 |
| Official Date: | 2013 |
| Dates: | Date Event 2013 Published |
| Volume: | Volume 8 |
| Number: | Number 3 |
| Page Range: | Article no. e58992 |
| DOI: | 10.1371/journal.pone.0058992 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Open Access (Creative Commons open licence) |
| Date of first compliant deposit: | 24 December 2015 |
| Date of first compliant Open Access: | 24 December 2015 |
| Funder: | National Science Foundation (U.S.) (NSF), University of Minnesota, Royal Society (Great Britain), United States. Dept. of Energy |
| Grant number: | IOB-0516811, IOB-0419648 (NSF), DE-FG02-05ER15670 (USDE) |
| URI: | https://wrap.warwick.ac.uk/53929/ |
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