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Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration
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Ratheesh, Aparna, Biebl, Julia, Vesela, Jana, Smutny, Michael, Papusheva, Ekaterina, Krens, S. F. Gabriel, Kaufmann, Walter, Gyoergy, Attila, Casano, Alessandra Maria and Siekhaus, Daria E. (2018) Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. Developmental Cell, 45 (3). pp. 331-346. e7. doi:10.1016/j.devcel.2018.04.002 ISSN 1534-5807.
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WRAP-drosophila-TNF-modulates-tissue-tension-embryo-facilitate-macrophage-invasive-migration-Smutny-2018.pdf - Accepted Version - Requires a PDF viewer. Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0. Download (4Mb) | Preview |
Official URL: http://dx.doi.org/10.1016/j.devcel.2018.04.002
Abstract
Migrating cells penetrate tissue barriers during development, inflammatory responses, and tumor metastasis. We study if migration in vivo in such three-dimensionally confined environments requires changes in the mechanical properties of the surrounding cells using embryonic Drosophila melanogaster hemocytes, also called macrophages, as a model. We find that macrophage invasion into the germband through transient separation of the apposing ectoderm and mesoderm requires cell deformations and reductions in apical tension in the ectoderm. Interestingly, the genetic pathway governing these mechanical shifts acts downstream of the only known tumor necrosis factor superfamily member in Drosophila, Eiger, and its receptor, Grindelwald. Eiger-Grindelwald signaling reduces levels of active Myosin in the germband ectodermal cortex through the localization of a Crumbs complex component, Patj (Pals-1-associated tight junction protein). We therefore elucidate a distinct molecular pathway that controls tissue tension and demonstrate the importance of such regulation for invasive migration in vivo.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QL Zoology Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Blood cells, Macrophages, Tumor necrosis factor, Drosophila, Tight junctions (Cell biology), Myosin | ||||||||||
Journal or Publication Title: | Developmental Cell | ||||||||||
Publisher: | Cell Press | ||||||||||
ISSN: | 1534-5807 | ||||||||||
Official Date: | 7 May 2018 | ||||||||||
Dates: |
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Volume: | 45 | ||||||||||
Number: | 3 | ||||||||||
Page Range: | pp. 331-346 | ||||||||||
Article Number: | e7 | ||||||||||
DOI: | 10.1016/j.devcel.2018.04.002 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Date of first compliant deposit: | 3 January 2019 | ||||||||||
Date of first compliant Open Access: | 3 January 2019 | ||||||||||
Funder: | Fonds zur Förderung der Wissenschaftlichen Forschung (Austria) (FWF) | ||||||||||
Grant number: | P_DASI_FWF01_P29638 | ||||||||||
RIOXX Funder/Project Grant: |
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