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Data for Synthetically scalable poly(ampholyte) which dramatically enhances cellular cryopreservation
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Bailey, Trisha L., Stubbs, Christopher, Murray, Kathryn, Tomás, Ruben M. F., Otten, Lucienne and Gibson, Matthew I. (2019) Data for Synthetically scalable poly(ampholyte) which dramatically enhances cellular cryopreservation. [Dataset]
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Official URL: http://wrap.warwick.ac.uk/123400
Abstract
The storage and transport of frozen cells underpin the emerging/existing cell-based therapies and are used in every biomedical research lab globally. The current gold-standard cryoprotectant dimethyl sulfoxide (DMSO) does not give quantitative cell recovery in suspension or in two-dimensional (2D) or three-dimensional (3D) cell models, and the solvent and cell debris must be removed prior to application/transfusion. There is a real need to improve this 50-year-old method to underpin emerging regenerative and cell-based therapies. Here, we introduce a potent and synthetically scalable polymeric cryopreservation enhancer which is easily obtained in a single step from a low cost and biocompatible precursor, poly(methyl vinyl ether-alt-maleic anhydride). This poly(ampholyte) enables post-thaw recoveries of up to 88% for a 2D cell monolayer model compared to just 24% using conventional DMSO cryopreservation. The poly(ampholyte) also enables reduction of [DMSO] from 10 wt % to just 2.5 wt % in suspension cryopreservation, which can reduce the negative side effects and speed up post-thaw processing. After thawing, the cells have reduced membrane damage and faster growth rates compared to those without the polymer. The polymer appears to function by a unique extracellular mechanism by stabilization of the cell membrane, rather than by modulation of ice formation and growth. This new macromolecular cryoprotectant will find applications across basic and translational biomedical science and may improve the cold chain for cell-based therapies.
Item Type: | Dataset | |||||||||||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||||||||
Type of Data: | Experimental data | |||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Cryopreservation of organs, tissues, etc., Cells -- Cryopreservation, Frozen erythrocytes, Polyampholytes | |||||||||||||||||||||
Journal or Publication Title: | Biomacromolecules | |||||||||||||||||||||
Publisher: | University of Warwick, Department of Chemistry | |||||||||||||||||||||
ISSN: | 1525-7797 | |||||||||||||||||||||
Official Date: | 31 July 2019 | |||||||||||||||||||||
Dates: |
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DOI: | 10.1021/acs.biomac.9b00681 | |||||||||||||||||||||
Status: | Not Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Media of Output (format): | .xlsx, .opj | |||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
Copyright Holders: | University of Warwick | |||||||||||||||||||||
Description: | Differential scanning calorimetry includes data used to calculate ice crystallization enthalpy and melting temperature, along with full DSC traces and is located in the “DSC” folder. |
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Date of first compliant deposit: | 5 July 2019 | |||||||||||||||||||||
Date of first compliant Open Access: | 11 March 2020 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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Contributors: |
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