Fedorenko, Olena A., Khovanov, Igor A., Roberts, Stephen K. and Guardiani, Carlo (2020) Data for Changes in ion selectivity following the asymmetrical addition of charge to the selectivity filter of bacterial sodium channels. [Dataset]

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Abstract

Voltage-gated sodium channels (NaVs) play fundamental roles in eukaryotes, but their exceptional size hinders their structural resolution. Bacterial NaVs are simplified homologues of their eukaryotic counterparts, but their use as models of eukaryotic Na+ channels is limited by their homotetrameric structure at odds with the asymmetric Selectivity Filter (SF) of eukaryotic NaVs. This work aims at mimicking the SF of eukaryotic NaVs by engineering radial asymmetry into the SF of bacterial channels. This goal was pursued with two approaches: the co-expression of different monomers of the NaChBac bacterial channel to induce the random assembly of heterotetramers, and the concatenation of four bacterial monomers to form a concatemer that can be targeted by site-specific mutagenesis. Patch-clamp measurements and Molecular Dynamics simulations showed that an additional gating charge in the SF leads to a significant increase in Na+ and a modest increase in the Ca2+ conductance in the NavMs concatemer in agreement with the behavior of the population of random heterotetramers with the highest proportion of channels with charge −5e. We thus showed that charge, despite being important, is not the only determinant of conduction and selectivity, and we created new tools extending the use of bacterial channels as models of eukaryotic counterparts.

Item Type:	Dataset
Subjects:	Q Science > QH Natural history Q Science > QP Physiology
Divisions:	Faculty of Science, Engineering and Medicine > Engineering > Engineering
Type of Data:	Experimental data
Library of Congress Subject Headings (LCSH):	Ion channels, Sodium channels, Eukaryotic cells
Publisher:	University of Warwick, School of Engineering
Official Date:	27 October 2020
Dates:	Date Event 27 October 2020 Created
Status:	Not Peer Reviewed
Publication Status:	Published
Media of Output (format):	.dat .xlsx .pdf
Access rights to Published version:	Open Access
Copyright Holders:	University of Warwick
Description:	Data record consists of a zip archive, containing the raw data in .dat and .xlsx format, and an accompanying data description PDF. Further information on individual data files can be found in the accompanying data description file.
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID EP/M015831/1 [EPSRC] Engineering and Physical Sciences Research Council http://dx.doi.org/10.13039/501100000266 EP/M016889/1 [EPSRC] Engineering and Physical Sciences Research Council http://dx.doi.org/10.13039/501100000266 803213 [ERC] Horizon 2020 Framework Programme http://dx.doi.org/10.13039/100010661
Related URLs:	Related item in WRAP Other
Contributors:	Contribution Name Contributor ID Depositor Khovanov, Igor A. 29260

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