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Opto-vTrap, an optogenetic trap for reversible inhibition of vesicular release, synaptic transmission, and behavior
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Won, Joungha, Pankratov, Yuriy, Jang, Minwoo Wendy, Kim, Sunpil, Ju, Yeon Ha, Lee, Sangkyu, Lee, Seung Eun, Kim, Arie, Park, Soowon, Lee, C. Justin and Heo, Won Do (2022) Opto-vTrap, an optogenetic trap for reversible inhibition of vesicular release, synaptic transmission, and behavior. Neuron, 110 (3). 423-435.e4. doi:10.1016/j.neuron.2021.11.003 ISSN 0896-6273.
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WRAP-opto-vTrap-optogenetic-trap-vesicular-release-synaptic-transmission-behavior-Pankratov-2022.pdf - Accepted Version - Requires a PDF viewer. Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0. Download (2172Kb) | Preview |
Official URL: https://doi.org/10.1016/j.neuron.2021.11.003
Abstract
Spatiotemporal control of brain activity by optogenetics has emerged as an essential tool to study brain function. For silencing brain activity, optogenetic probes, such as halorhodopsin and archaerhodopsin, inhibit transmitter release indirectly by hyperpolarizing membrane potentials. However, these probes cause an undesirable ionic imbalance and rebound spikes. Moreover, they are not applicable to use in non-excitable glial cells. Here we engineered Opto-vTrap, a light-inducible and reversible inhibition system to temporarily trap the transmitter-containing vesicles from exocytotic release. Light activation of Opto-vTrap caused full vesicle clusterization and complete inhibition of exocytosis within 1 min, which recovered within 30 min after light off. We found a significant reduction in synaptic and gliotransmission upon activation of Opto-vTrap in acute brain slices. Opto-vTrap significantly inhibited hippocampus-dependent memory retrieval with full recovery within an hour. We propose Opto-vTrap as a next-generation optogenetic silencer to control brain activity and behavior with minimal confounding effects.
| Item Type: | Journal Article | ||||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||
| SWORD Depositor: | Library Publications Router | ||||||||||
| Library of Congress Subject Headings (LCSH): | Brain -- Physiology, Optogenetics , Exocytosis | ||||||||||
| Journal or Publication Title: | Neuron | ||||||||||
| Publisher: | Cell Press | ||||||||||
| ISSN: | 0896-6273 | ||||||||||
| Official Date: | 2 February 2022 | ||||||||||
| Dates: |
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| Volume: | 110 | ||||||||||
| Number: | 3 | ||||||||||
| Page Range: | 423-435.e4 | ||||||||||
| DOI: | 10.1016/j.neuron.2021.11.003 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||
| Date of first compliant deposit: | 12 April 2022 | ||||||||||
| Date of first compliant Open Access: | 22 November 2022 |
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