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Genome‐wide dFOXO targets and topology of the transcriptomic response to stress and insulin signalling
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Alic, Nazif, Andrews, T. Daniel, Giannakou, Maria E., Papatheodorou, Irene, Slack, Cathy, Hoddinott, Matthew P., Cochemé, Helena M., Schuster, Eugene F., Thornton, Janet M. and Partridge, Linda (2011) Genome‐wide dFOXO targets and topology of the transcriptomic response to stress and insulin signalling. Molecular Systems Biology, 7 (1). 502. doi:10.1038/msb.2011.36 ISSN 1744-4292.
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Official URL: http://doi.org/10.1038/msb.2011.36
Abstract
FoxO transcription factors, inhibited by insulin/insulin-like growth factor signalling (IIS), are crucial players in numerous organismal processes including lifespan. Using genomic tools, we uncover over 700 direct dFOXO targets in adult female Drosophila. dFOXO is directly required for transcription of several IIS components and interacting pathways, such as TOR, in the wild-type fly. The genomic locations occupied by dFOXO in adults are different from those observed in larvae or cultured cells. These locations remain unchanged upon activation by stresses or reduced IIS, but the binding is increased and additional targets activated upon genetic reduction in IIS. We identify the part of the IIS transcriptional response directly controlled by dFOXO and the indirect effects and show that parts of the transcriptional response to IIS reduction do not require dfoxo. Promoter analyses revealed GATA and other forkhead factors as candidate mediators of the indirect and dfoxo-independent effects. We demonstrate genome-wide evolutionary conservation of dFOXO targets between the fly and the worm Caenorhabditis elegans, enriched for a second tier of regulators including the dHR96/daf-12 nuclear hormone receptor.
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Drosophila, Drosophila -- Physiology, Somatomedin , Growth factors , Cells -- Growth | ||||||||||||||||||
Journal or Publication Title: | Molecular Systems Biology | ||||||||||||||||||
Publisher: | Nature Publishing Group | ||||||||||||||||||
ISSN: | 1744-4292 | ||||||||||||||||||
Official Date: | 1 January 2011 | ||||||||||||||||||
Dates: |
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Volume: | 7 | ||||||||||||||||||
Number: | 1 | ||||||||||||||||||
Article Number: | 502 | ||||||||||||||||||
DOI: | 10.1038/msb.2011.36 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Date of first compliant deposit: | 18 May 2023 | ||||||||||||||||||
Date of first compliant Open Access: | 18 May 2023 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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