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A new membrane protein Sbg1 links the contractile ring apparatus and septum synthesis machinery in fission yeast
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Sethi, Kriti, Palani, Saravanan, Cortés, Juan C G, Sato, Mamiko, Sevugan, Mayalagu, Ramos, Mariona, Vijaykumar, Shruthi, Osumi, Masako, Naqvi, Naweed I, Ribas, Juan Carlos and Balasubramanian, Mohan K. (2016) A new membrane protein Sbg1 links the contractile ring apparatus and septum synthesis machinery in fission yeast. PLoS Genetics, 12 (10). e1006383. doi:10.1371/journal.pgen.1006383
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Official URL: http://dx.doi.org/10.1371/journal.pgen.1006383
Abstract
Cytokinesis in many organisms requires a plasma membrane anchored actomyosin ring, whose contraction facilitates cell division. In yeast and fungi, actomyosin ring constriction is also coordinated with division septum assembly. How the actomyosin ring interacts with the plasma membrane and the plasma membrane-localized septum synthesizing machinery remains poorly understood. In Schizosaccharomyces pombe, an attractive model organism to study cytokinesis, the β-1,3-glucan synthase Cps1p / Bgs1p, an integral membrane protein, localizes to the plasma membrane overlying the actomyosin ring and is required for primary septum synthesis. Through a high-dosage suppressor screen we identified an essential gene, sbg1+ (suppressor of beta glucan synthase 1), which suppressed the colony formation defect of Bgs1-defective cps1-191 mutant at higher temperatures. Sbg1p, an integral membrane protein, localizes to the cell ends and to the division site. Sbg1p and Bgs1p physically interact and are dependent on each other to localize to the division site. Loss of Sbg1p results in an unstable actomyosin ring that unravels and slides, leading to an inability to deposit a single contiguous division septum and an important reduction of the β-1,3-glucan proportion in the cell wall, coincident with that observed in the cps1-191 mutant. Sbg1p shows genetic and / or physical interaction with Rga7p, Imp2p, Cdc15p, and Pxl1p, proteins known to be required for actomyosin ring integrity and efficient septum synthesis. This study establishes Sbg1p as a key member of a group of proteins that link the plasma membrane, the actomyosin ring, and the division septum assembly machinery in fission yeast.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||||||
| SWORD Depositor: | Library Publications Router | ||||||||
| Library of Congress Subject Headings (LCSH): | Cell division, Cell membranes, Actomyosin | ||||||||
| Journal or Publication Title: | PLoS Genetics | ||||||||
| Publisher: | Public Library of Science | ||||||||
| ISSN: | 1553-7390 | ||||||||
| Official Date: | 17 October 2016 | ||||||||
| Dates: |
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| Volume: | 12 | ||||||||
| Number: | 10 | ||||||||
| Article Number: | e1006383 | ||||||||
| DOI: | 10.1371/journal.pgen.1006383 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access | ||||||||
| Funder: | Temasek Lifesciences Laboratory , Wellcome Trust (London, England), Royal Society (Great Britain). Wolfson Research Merit Award (RSWRMA), Spain. Ministerio de Ciencia e Innovación (MICINN), Nihon Joshi Daigaku [Japan women's university] (JWU), Japan. Monbu Kagakushō [Japan. Ministry of Education, Culture, Sports, Science and Technology] (MEXT) | ||||||||
| Grant number: | BIO2012-35372, BIO2015- 69958-P (MICINN), S0991205 (JWU) | ||||||||
| Adapted As: |
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