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A stirring system using suspended magnetically-actuated pillars for controlled cell clustering
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Saberi, Aref, Zhang, Shuaizhong, van den Bersselaar, Carola, Kandail, Harkamaljot S., den Toonder, Jaap M. J. and Kurniawan, Nicholas A. (2019) A stirring system using suspended magnetically-actuated pillars for controlled cell clustering. Soft Matter, 15 (6). pp. 1435-1443. doi:10.1039/C8SM01957F ISSN 1744-683X.
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Official URL: http://dx.doi.org/10.1039/C8SM01957F
Abstract
Controlled stirring of a solution is a household task in most laboratories. However, most stirring methods are perturbative or require vessels with predefined shapes and sizes. Here we propose a novel stirring system based on suspended magnetically-actuated pillars (SMAPs), inspired by the ability of biological flagella and cilia to generate flow. We fabricated flexible, millimeter-scale magnetic pillars grafted on transparent polydimethylsiloxane (PDMS) substrates and built a simple actuation setup to control the motion of the pillars remotely. We tested the system with a standard 24-well plate routinely used in most research laboratories and demonstrate that the magnetic actuation results in robust bending of the pillars and large-scale fluid flow in the wells. Quantitative analysis using computational fluid dynamics modeling indicates that the flow profile in the well can be tuned by modulating the applied magnetic field and the geometries of the well and the pillar. Finally, we show that, by employing the stirring system in a standard cell culture plate, we were able to obtain controlled clustering of cells. The SMAP stirring system is therefore a promising cost-effective and scalable stirring approach for various types of studies involving colloids as well as soft and biological materials.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QH Natural history Q Science > QP Physiology T Technology > TA Engineering (General). Civil engineering (General) |
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Divisions: | Faculty of Science, Engineering and Medicine > Engineering > WMG (Formerly the Warwick Manufacturing Group) | ||||||||
Library of Congress Subject Headings (LCSH): | Mixing, Computational fluid dynamics, Fluid dynamics, Biomimetics, Cell culture -- Technique | ||||||||
Journal or Publication Title: | Soft Matter | ||||||||
Publisher: | Royal Society of Chemistry | ||||||||
ISSN: | 1744-683X | ||||||||
Official Date: | 2019 | ||||||||
Dates: |
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Volume: | 15 | ||||||||
Number: | 6 | ||||||||
Page Range: | pp. 1435-1443 | ||||||||
DOI: | 10.1039/C8SM01957F | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 9 January 2020 | ||||||||
Date of first compliant Open Access: | 17 January 2020 |
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