Carver, Mark A. (1979) Studies on lipid involvement in the mechanism of energy coupling. PhD thesis, University of Warwick.

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Abstract

The interactions of derivatives of lipoic acid and unsaturated fatty acids with the mitochondrial energy coupling system was investigated. Derivatives of dihydrolipoic acid, oleoyl-S-lipoate and oleoyl phosphate were observed to stimulate ATP synthesis in a number of ATPase preparations derived from bovine heart and other mitochondria.

This synthesis was sensitive to various inhibitors of oxidative phosphorylation such as oligomycin, efrapeptin, triorganotin halides and uncoupling agents.

A derivative of dihydrolipoic acid also stimulated an energy-linked transhydrogenase reaction in bovine heart SMP. This reaction has features in common with both ATP and respiration-driven transhydrogenase.

All these reactions were observed at a low frequency. The reasons for this were investigated as were the precise natures of the compounds stimulating ATP synthesis. No clear answers were obtained to either question, although suggestions were made.

The role of ubiquinone in energy coupling was also investigated, using solvent extraction studies and a low molecular weight homologue of ubiquinone, ubiquinone 3. These studies suggest that ubiquinone may have a role in energy coupling reactions other than that expected from its well-know role as a respiratory chain redox carrier, possibly in the control of various ATPase-linked reactions, e.g. ATP synthesis, ATP—Pi exchange, ATP-driven transhydrogenase.

The results presented here describing 'dihydrolipoate' driven ATP synthesis (tables 3.1-3.19 & fig.3.2) and oleoyl phosphate driven ATP synthesis and ATP-Pi exchange ( tables 5.3-5.10 & figs.5.1-5.7 ) were most of those obtained showing these reactions over a two year period and represent 'v 1% of the total number of attempts to demonstrate them (see pp 88-89,212-213).

The remaining results presented in this thesis are usually representative of 2-6 experiments performed in duplicate.

Item Type:	Thesis (PhD)
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history Q Science > QP Physiology
Library of Congress Subject Headings (LCSH):	Lipoic acid, Unsaturated fatty acids, Mitochondria
Official Date:	January 1979
Dates:	Date Event January 1979 Submitted
Institution:	University of Warwick
Theses Department:	Department of Chemistry and Molecular Sciences
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Griffiths, D. E.
Extent:	xv, 360 leaves : illustrations, charts
Language:	eng

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