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Golgi-localized putative S-adenosyl methionine transporters required for plant cell wall polysaccharide methylation
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Temple, Henry, Phyo, Pyae, Yang, Weibing, Lyczakowski, Jan J., Echevarría-Poza, Alberto, Yakunin, Igor, Parra-Rojas, Juan Pablo, Terrett, Oliver M., Saez-Aguayo, Susana, Dupree, Ray, Orellana, Ariel, Hong, Mei and Dupree, Paul (2022) Golgi-localized putative S-adenosyl methionine transporters required for plant cell wall polysaccharide methylation. Nature Plants, 8 (6). pp. 656-669. doi:10.1038/s41477-022-01156-4 ISSN 2055-026X.
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WRAP-golgi-localized-putative-S-adenosyl-methionine-transporters-required-plant-cell-wall-polysaccharide-methylation-Dupree-2022.pdf - Accepted Version - Requires a PDF viewer. Download (4Mb) | Preview |
Official URL: https://doi.org/10.1038/s41477-022-01156-4
Abstract
Polysaccharide methylation, especially that of pectin, is a common and important feature of land plant cell walls. Polysaccharide methylation takes place in the Golgi apparatus and therefore relies on the import of S-adenosyl methionine (SAM) from the cytosol into the Golgi. However, so far, no Golgi SAM transporter has been identified in plants. Here we studied major facilitator superfamily members in Arabidopsis that we identified as putative Golgi SAM transporters (GoSAMTs). Knockout of the two most highly expressed GoSAMTs led to a strong reduction in Golgi-synthesized polysaccharide methylation. Furthermore, solid-state NMR experiments revealed that reduced methylation changed cell wall polysaccharide conformations, interactions and mobilities. Notably, NMR revealed the existence of pectin 'egg-box' structures in intact cell walls and showed that their formation is enhanced by reduced methyl esterification. These changes in wall architecture were linked to substantial growth and developmental phenotypes. In particular, anisotropic growth was strongly impaired in the double mutant. The identification of putative transporters involved in import of SAM into the Golgi lumen in plants provides new insights into the paramount importance of polysaccharide methylation for plant cell wall structure and function.
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history Q Science > QK Botany Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Physics | ||||||||||||||||||
SWORD Depositor: | Library Publications Router | ||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Plant cells and tissues, Plant cell walls, Polysaccharides, Methylation, Pectin, Adenosylmethionine, Cytosol, Golgi apparatus | ||||||||||||||||||
Journal or Publication Title: | Nature Plants | ||||||||||||||||||
Publisher: | Nature Publishing Group | ||||||||||||||||||
ISSN: | 2055-026X | ||||||||||||||||||
Official Date: | June 2022 | ||||||||||||||||||
Dates: |
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Volume: | 8 | ||||||||||||||||||
Number: | 6 | ||||||||||||||||||
Page Range: | pp. 656-669 | ||||||||||||||||||
DOI: | 10.1038/s41477-022-01156-4 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Reuse Statement (publisher, data, author rights): | This version of the article has been accepted for publication, after peer review (when applicable) but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1038/s41477-022-01156-4. Use of this Accepted Version is subject to the publisher’s Accepted Manuscript terms of use https://www.springernature.com/gp/open research/policies/acceptedmanuscript-terms. | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Date of first compliant deposit: | 29 June 2022 | ||||||||||||||||||
Date of first compliant Open Access: | 9 December 2022 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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