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Expression and purification of recombinant G protein-coupled receptors : a review
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Wiseman, Daniel N., Otchere, Abigail, Patel, Jaimin H., Uddin, Romez, Pollock, Naomi L., Routledge, Sarah J., Rothnie, Alice J., Slack, Cathy, Poyner, David R., Bill, Roslyn M. and Goddard, Alan D. (2020) Expression and purification of recombinant G protein-coupled receptors : a review. Protein Expression and Purification, 167 . 105524. doi:10.1016/j.pep.2019.105524 ISSN 1046-5928.
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Official URL: http://doi.org/10.1016/j.pep.2019.105524
Abstract
Given their extensive role in cell signalling, GPCRs are significant drug targets; despite this, many of these receptors have limited or no available prophylaxis. Novel drug design and discovery significantly rely on structure determination, of which GPCRs are typically elusive. Progress has been made thus far to produce sufficient quantity and quality of protein for downstream analysis. As such, this review highlights the systems available for recombinant GPCR expression, with consideration of their advantages and disadvantages, as well as examples of receptors successfully expressed in these systems. Additionally, an overview is given on the use of detergents and the styrene maleic acid (SMA) co-polymer for membrane solubilisation, as well as purification techniques.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | G proteins, G proteins -- Receptors, Cellular signal transduction, Cell interaction, Microcrystalline polymers | ||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Protein Expression and Purification | ||||||||||||||||||||||||||||||||||||
Publisher: | Academic Press | ||||||||||||||||||||||||||||||||||||
ISSN: | 1046-5928 | ||||||||||||||||||||||||||||||||||||
Official Date: | March 2020 | ||||||||||||||||||||||||||||||||||||
Dates: |
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Volume: | 167 | ||||||||||||||||||||||||||||||||||||
Article Number: | 105524 | ||||||||||||||||||||||||||||||||||||
DOI: | 10.1016/j.pep.2019.105524 | ||||||||||||||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||||||||||||||
Date of first compliant deposit: | 18 May 2023 | ||||||||||||||||||||||||||||||||||||
Date of first compliant Open Access: | 18 May 2023 | ||||||||||||||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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