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Single-molecule FRET for virology : 20 years of insight into protein structure and dynamics
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Groves, Danielle, Hepp, Christof, Kapanidis, Achillefs N. and Robb, Nicole C. (2023) Single-molecule FRET for virology : 20 years of insight into protein structure and dynamics. Quarterly Reviews of Biophysics, 56 . e3. doi:10.1017/S0033583523000021 ISSN 0033-5835.
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Official URL: http://dx.doi.org/10.1017/S0033583523000021
Abstract
Although viral protein structure and replication mechanisms have been explored extensively with X-ray crystallography, cryo-electron microscopy, and population imaging studies, these methods are often not able to distinguish dynamic conformational changes in real time. Single-molecule fluorescence resonance energy transfer (smFRET) offers unique insights into interactions and states that may be missed in ensemble studies, such as nucleic acid or protein structure, and conformational transitions during folding, receptor–ligand interactions, and fusion. We discuss the application of smFRET to the study of viral protein conformational dynamics, with a particular focus on viral glycoprotein dynamics, viral helicases, proteins involved in HIV reverse transcription, and the influenza RNA polymerase. smFRET experiments have played a crucial role in deciphering conformational changes in these processes, emphasising the importance of smFRET as a tool to help elucidate the life cycle of viral pathogens and identify key anti-viral targets.
Item Type: | Journal Article | |||||||||||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QP Physiology Q Science > QR Microbiology R Medicine > RA Public aspects of medicine |
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Fluorescence microscopy, Proteins -- Structure , Molecular virology, Receptor-ligand complexes, COVID-19 (Disease) , Molecular biology -- Technique | |||||||||||||||||||||
Journal or Publication Title: | Quarterly Reviews of Biophysics | |||||||||||||||||||||
Publisher: | Cambridge University Press | |||||||||||||||||||||
ISSN: | 0033-5835 | |||||||||||||||||||||
Official Date: | 2023 | |||||||||||||||||||||
Dates: |
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Volume: | 56 | |||||||||||||||||||||
Article Number: | e3 | |||||||||||||||||||||
DOI: | 10.1017/S0033583523000021 | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||||||||
Date of first compliant deposit: | 11 July 2023 | |||||||||||||||||||||
Date of first compliant Open Access: | 12 July 2023 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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