Smith, Ciearra B., van der Vinne, Vincent, McCartney, Eleanor, Stowie, Adam C., Leise, Tanya L., Blanca, Martin-Burgos, Molyneux, Penny C., Garbutt, Lauren A, Brodsky, Michael H., Davidson, Alec J., Harrington, Mary E., Dallmann, Robert and Weaver, David R. (2022) Cell-type specific circadian bioluminescence rhythms in Dbp reporter mice. Journal of Biological Rhythms, 37 (1). pp. 53-77. doi:10.11770748/7304211069452 ISSN 0748-7304.
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Abstract
Circadian rhythms are endogenously generated physiological and molecular rhythms with a cycle length of about 24 h. Bioluminescent reporters have been exceptionally useful for studying circadian rhythms in numerous species. Here, we report development of a reporter mouse generated by modification of a widely expressed and highly rhythmic gene encoding D-site albumin promoter binding protein (Dbp). In this line of mice, firefly luciferase is expressed from the Dbp locus in a Cre recombinase-dependent manner, allowing assessment of bioluminescence rhythms in specific cellular populations. A mouse line in which luciferase expression was Cre-independent was also generated. The Dbp reporter alleles do not alter Dbp gene expression rhythms in liver or circadian locomotor activity rhythms. In vivo and ex vivo studies show the utility of the reporter alleles for monitoring rhythmicity. Our studies reveal cell-type-specific characteristics of rhythms among neuronal populations within the suprachiasmatic nuclei ex vivo. In vivo studies show Dbp-driven bioluminescence rhythms in the liver of Albumin-Cre;DbpKI/+ “liver reporter” mice. After a shift of the lighting schedule, locomotor activity achieved the proper phase relationship with the new lighting cycle more rapidly than hepatic bioluminescence did. As previously shown, restricting food access to the daytime altered the phase of hepatic rhythmicity. Our model allowed assessment of the rate of recovery from misalignment once animals were provided with food ad libitum. These studies confirm the previously demonstrated circadian misalignment following environmental perturbations and reveal the utility of this model for minimally invasive, longitudinal monitoring of rhythmicity from specific mouse tissues.
| Item Type: | Journal Article |
|---|---|
| Alternative Title: | |
| Subjects: | Q Science > QH Natural history Q Science > QL Zoology Q Science > QP Physiology |
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
| Library of Congress Subject Headings (LCSH): | Circadian rhythms, Bioluminescence , Luciferases , Albumins , Cell cycle , Mice as laboratory animals , Biological rhythms -- Mathematical models |
| Journal or Publication Title: | Journal of Biological Rhythms |
| Publisher: | Sage Publications Ltd. |
| ISSN: | 0748-7304 |
| Official Date: | 1 February 2022 |
| Dates: | Date Event 1 February 2022 Published 13 January 2022 Available 6 December 2021 Accepted |
| Volume: | 37 |
| Number: | 1 |
| Page Range: | pp. 53-77 |
| DOI: | 10.11770748/7304211069452 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Re-use Statement: | Smith, Ciearra B., van der Vinne, Vincent, McCartney, Eleanor, Stowie, Adam C., Leise, Tanya L., Blanca, Martin-Burgos, Molyneux, Penny C., Garbutt, Lauren A., Brodsky, Michael H., Davidson, Alec J., Harrington, Mary E., Dallmann, Robert and Weaver, David R. (2022) Cell-type specific circadian bioluminescence rhythms in Dbp reporter mice. Journal of Biological Rhythms. 37(1) pp.53-77. Copyright © 2022 (Copyright Holder). Reprinted by permission of SAGE Publications. DOI: https://doi.org/10.11770748/7304211069452 Users who receive access to an article through a repository are reminded that the article is protected by copyright and reuse is restricted to non-commercial and no derivative uses. |
| Access rights to Published version: | Restricted or Subscription Access |
| Date of first compliant deposit: | 21 December 2021 |
| Date of first compliant Open Access: | 4 January 2022 |
| RIOXX Funder/Project Grant: | Project/Grant ID RIOXX Funder Name Funder ID UNSPECIFIED Hartmann Müller-Stiftung für Medizinische Forschung |
| Related URLs: | |
| URI: | https://wrap.warwick.ac.uk/161349/ |
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