Rodrigo, Guillermo, Prakash, Satya, Cordero, Teresa, Kushwaha, Manish and Jaramillo, Alfonso (2016) Functionalization of an antisense small RNA. Journal of Molecular Biology, 428 Part B (5). pp. 889-892. doi:10.1016/j.jmb.2015.12.022 ISSN 0022-2836.
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Abstract
In order to explore the possibility of adding new functions to preexisting genes, we considered a framework of riboregulation. We created a new riboregulator consisting of the reverse complement of a known riboregulator. Using computational design, we engineered a cis-repressing 5' untranslated region that can be activated by this new riboregulator. As a result, both RNAs can orthogonally trans-activate translation of their cognate, independent targets. The two riboregulators can also repress each other by antisense interaction, although not symmetrically. Our work highlights that antisense small RNAs can work as regulatory agents beyond the antisense paradigm, and that they could hence be interfaced with other circuits used in synthetic biology.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH426 Genetics |
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
| Library of Congress Subject Headings (LCSH): | RNA -- Structure, RNA -- Analysis, Life sciences, Computer-aided engineering, Neural networks, Riboswitches -- Computer simulation, Biotechnology |
| Journal or Publication Title: | Journal of Molecular Biology |
| Publisher: | Academic Press |
| ISSN: | 0022-2836 |
| Official Date: | 4 January 2016 |
| Dates: | Date Event 4 January 2016 Available 23 December 2015 Accepted 15 May 2015 Submitted |
| Volume: | 428 Part B |
| Number: | 5 |
| Page Range: | pp. 889-892 |
| DOI: | 10.1016/j.jmb.2015.12.022 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Date of first compliant deposit: | 13 January 2016 |
| Date of first compliant Open Access: | 23 June 2016 |
| Related URLs: | |
| URI: | https://wrap.warwick.ac.uk/75645/ |
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