Likhitsup, Asawin, Deeth, Robert J., Otto, Sijbren and Marsh, Andrew (2009) Apparent non-statistical binding in a ditopic receptor for guanosine. Organic & Biomolecular Chemistry, Vol.7 (No.10). pp. 2093-2103. doi:10.1039/b812969j ISSN 1477-0520.
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Abstract
Analysis of stepwise association constants for guests binding to more than one site in a receptor is expected to give a ratio of the first association constant to the second of about 4 : 1 on statistical grounds (since a second guest should have an equal chance of binding to a different site on the same, or a new molecule). Taking account of self-association in our analysis of a system in which the binding sites are close together, we observe a ratio closer to 1 : 1, indicative of non-statistical, or cooperative binding. The longer homologue built around two alkynes displays a very different ratio of stepwise association constants of about 7 : 1. We discuss the origins of this unusual behaviour in terms of steric interactions within the receptors and their corresponding complexes with guanosine derivatives.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry |
| Library of Congress Subject Headings (LCSH): | Binding sites (Biochemistry) |
| Journal or Publication Title: | Organic & Biomolecular Chemistry |
| Publisher: | Royal Society of Chemistry |
| ISSN: | 1477-0520 |
| Official Date: | 2009 |
| Dates: | Date Event 2009 Published |
| Volume: | Vol.7 |
| Number: | No.10 |
| Number of Pages: | 11 |
| Page Range: | pp. 2093-2103 |
| DOI: | 10.1039/b812969j |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Description: | Attached MS Excel spreadsheets that enable curve fitting of NMR titration data and calculation of probabilities of binding for molecules above. Monoalkyne is receptor 1, dialkyne is receptor 2, guanosine is guest 3. Also attached are PDF files of Supporting Information here including methods used for NMR and ITC titrations and analysis. |
| Date of first compliant deposit: | 13 December 2015 |
| Date of first compliant Open Access: | 13 December 2015 |
| Funder: | University of Warwick. Dept. of Chemistry |
| URI: | https://wrap.warwick.ac.uk/27963/ |
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